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Mutated coronavirus binds up to seven times stronger to human receptor

Mutations in the spike protein of the coronavirus, cause up to seven times stronger binding to the receptor on our cells. This was discovered by researchers at Erasmus MC after analyzing coronavirus variants that were first described in England and South Africa. The increase in binding affinity possibly contributes to the faster spread of these variants.

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The variant of coronavirus identified in England, popularly called the British variant, binds seven times more strongly to the so-called ACE2 receptor on human cells than the original virus from Wuhan. The “South African variant” binds three times stronger. This is the conclusion of researchers from the Department of Molecular Genetics led by biochemist Dr. Joyce Lebbink, in the scientific Journal of Molecular Biology .

Only a few atoms

A seven-fold increase in binding is a big difference, Lebbink believes. ‘You have to realize that we are talking about one amino acid change between the original variant and the variant identified in England. This is only a few atoms causing such a big difference.’

Mutations in the spike protein of the virus (blue spheres in grey strands) affect the binding to the ACE2 receptor on the body cells (orange).

Lebbink believes the stronger binding may contribute to the faster spread of the coronavirus mutants. ‘Attachment of the virus to our cells is not the only factor that determines infectivity, but from a biochemical point of view this correlation does not surprise us. These virus variants bind longer to the receptor than the original virus and therefore have a greater chance of entering the cell.’

Affinity

The research involved a feat of genetic and technological ingenuity. Using synthetic DNA, the researchers recreated pieces of the spike protein of the coronavirus variants and the human ACE2 receptor to which the virus binds. By injecting a solution containing the pieces of spike protein over a chip with a layer of ACE2 receptors, the scientists were able to determine how quickly the spike protein binds to the receptor and how rapidly it releases. Combined, this determines affinity, or the strength of binding. By repeating the experiment with the different virus variants, an accurate comparison of binding affinities could be made.

DNA repair

Lebbink and her colleagues Charlie Laffeber, Kelly de Koning and Prof Roland Kanaar normally work on DNA repair. A completely different field from coronaviruses. ‘It’s an excursion, initiated during the first lockdown. Like many other scientists we felt the need to contribute,’ says Lebbink. The technique they use to measure, for example, how mutated proteins bind to DNA proved equally applicable to the interaction between coronavirus and ACE2 receptor. Meanwhile, normal work on DNA repair has largely resumed, but there are plans to also determine the binding affinity of the variant causing problems in India.